abstract：:Bacteriophage phi 29 DNA polymerase is sensitive to aphidicolin (APH). DNA polymerase of the APH-resistant mutant, APHr71, was more sensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'triphosphate than the wild type. Nucleotide sequence analysis revealed a single transition of G at nucleotide 562 to A in...

journal_title：Virology

authors： Matsumoto K,Kim CI,Kobayashi H,Kanehiro H,Hirokawa H

更新日期：1990-09-01 00:00:00

abstract：:We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...

journal_title：Virology

authors： Kondo K,Bando H,Tsurudome M,Kawano M,Nishio M,Ito Y

更新日期：1990-09-01 00:00:00

abstract：:Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...

journal_title：Virology

authors： Campadelli-Fiume G,Stirpe D,Boscaro A,Avitabile E,Foá-Tomasi L,Barker D,Roizman B

更新日期：1990-09-01 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...

journal_title：Virology

authors： Godeny EK,Speicher DW,Brinton MA

更新日期：1990-08-01 00:00:00

abstract：:The mechanism of trans-acting regulation of transcription from the long terminal repeat (LTR) of human immunodeficiency virus (HIV) has been investigated. The roles of the cis-acting elements within HIV LTR, as well as the trans-acting factors present in HIV-infected cells, have been evaluated by an in vitro transcrip...

journal_title：Virology

authors： Okamoto T,Benter T,Josephs SF,Sadaie MR,Wong-Staal F

更新日期：1990-08-01 00:00:00

abstract：:The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...

journal_title：Virology

authors： Air GM,Laver WG,Luo M,Stray SJ,Legrone G,Webster RG

更新日期：1990-08-01 00:00:00

abstract：:Barley stripe mosaic virus (BSMV) has a tripartite genome comprising RNAs designated alpha, beta, and gamma, which collectively encode seven polypeptides. We show here that an antiserum raised against an abundant disease-specific protein from BSMV-infected plants reacts specifically with the viral beta b gene product ...

journal_title：Virology

authors： Petty IT,Jackson AO

更新日期：1990-08-01 00:00:00

abstract：:A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...

journal_title：Virology

authors： Matsukura T,Sugase M

更新日期：1990-08-01 00:00:00

abstract：:We report the construction of herpes simplex virus 1 recombinants from which all or part of the coding sequences of four open reading frames have been deleted. In recombinants R7101 and R7108, the BamHI D' fragment containing the coding sequences of the dUTPase gene and the promoter-regulatory domain of a late gene wa...

journal_title：Virology

authors： Barker DE,Roizman B

更新日期：1990-08-01 00:00:00

abstract：:The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...

journal_title：Virology

authors： Simon KO,Cardamone JJ Jr,Whitaker-Dowling PA,Youngner JS,Widnell CC

更新日期：1990-07-01 00:00:00

abstract：:Woodchuck hepatitis core antigen (WHcAg) particles purified from the liver of chronically infected animals were used for monoclonal antibody production. Most of the putative clones demonstrated anti-WHc specificity. However, the supernatants from several putative clones bound X antigen sequences from woodchuck hepatit...

journal_title：Virology

authors： Feitelson MA,Clayton MM,Phimister B

更新日期：1990-07-01 00:00:00

abstract：:The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...

journal_title：Virology

authors： Hearne PQ,Knorr DA,Hillman BI,Morris TJ

更新日期：1990-07-01 00:00:00

abstract：:Human hepatitis B virus (HBV) X-gene, previously shown to be capable of trans-activating heterologous regulatory elements of the human beta-interferon gene, the human immunodeficiency virus type I (HIV-1) long terminal repeat (LTR), the simian virus 40 (SV40), and HBV, has the capacity to code for a 17-kDa polypeptide...

journal_title：Virology

authors： Twu JS,Wu JY,Robinson WS

更新日期：1990-07-01 00:00:00

abstract：:The nucleotide sequence of the leader and the gene encoding the nucleocapsid protein (NP) of mumps virus Miyahara strain have been determined. The leader sequence is 55 nucleotides in length and the NP gene is 1845 nucleotides in length, exclusive of poly(A). The NP gene codes for a protein of 549 amino acids, with a ...

journal_title：Virology

authors： Tanabayashi K,Takeuchi K,Hishiyama M,Yamada A,Tsurudome M,Ito Y,Sugiura A

更新日期：1990-07-01 00:00:00

abstract：:Neutralizing sites on bluetongue virus serotype 10 (BTV-10) were investigated with a panel of seven neutralizing monoclonal antibodies (MAbs). Each MAb was coupled to agarose beads and tested against the other MAbs and a nonneutralizing control MAb in a competitive immueprecipitation assay. In addition, neutralization...

journal_title：Virology

authors： Heidner HW,Rossitto PV,MacLachlan NJ

更新日期：1990-06-01 00:00:00

abstract：:The hemagglutinin-neuraminidase (HN) gene of the Hitchner B1 strain of Newcastle disease virus (NDV) was cloned as a cDNA and inserted into a baculovirus expression vector. The recombinant HN (recHN) expressed in Spodoptera frugiperda cells had both hemagglutinating and neuraminidase activities both of which were inhi...

journal_title：Virology

authors： Nagy E,Derbyshire JB,Dobos P,Krell PJ

更新日期：1990-06-01 00:00:00

abstract：:The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...

journal_title：Virology

authors： Fox E,Moen PT Jr,Bodnar JW

更新日期：1990-06-01 00:00:00

abstract：:Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...

journal_title：Virology

authors： Petti L,Sample C,Kieff E

更新日期：1990-06-01 00:00:00

abstract：:In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...

journal_title：Virology

authors： Rando RF,Srinivasan A,Feingold J,Gonczol E,Plotkin S

更新日期：1990-05-01 00:00:00

abstract：:The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed co...

journal_title：Virology

authors： Lanford RE,Notvall L

更新日期：1990-05-01 00:00:00

abstract：:The effect of 2-aminopurine (2AP), an inhibitor of the RNA-dependent P1/eIF-2 protein kinase, on the expression of the reovirus serotype 1 Lang strain S1 and S4 genes in transfected simian COS cells was examined. In the absence of 2AP, the s4-encoded sigma 3 gene product was expressed about five times more efficiently...

journal_title：Virology

authors： Samuel CE,Brody MS

更新日期：1990-05-01 00:00:00

abstract：:The group B noncultivatable rotavirus, ADRV, was purified from infected stool specimens. Double-stranded RNA was extracted, polyadenylated, reverse transcribed into cDNA, and cloned into plasmid vector pAT153. Each cDNA clone hybridized to a single ADRV RNA segment and cDNA clones of each genomic RNA segment were iden...

journal_title：Virology

authors： Chen GM,Hung T,Mackow ER

更新日期：1990-04-01 00:00:00

abstract：:After intranasal inoculation, mouse hepatitis virus (MHV) gains entry into the central nervous system (CNS) via the olfactory and trigeminal nerves. Under the appropriate conditions, some mice develop clinically apparent demyelinating encephalomyelitis several weeks later, with virus always present in the spinal cord....

journal_title：Virology

authors： Perlman S,Jacobsen G,Olson AL,Afifi A

更新日期：1990-04-01 00:00:00

abstract：:The effect of rev (art/trs) gene on the level of HIV-1 envelope (env) expression using recombinant adenovirus was investigated. Recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (HIV-1) were constructed by inserting the gene into an expression cassette. ...

journal_title：Virology

authors： Chanda PK,Natuk RJ,Mason BB,Bhat BM,Greenberg L,Dheer SK,Molnar-Kimber KL,Mizutani S,Lubeck MD,Davis AR

更新日期：1990-04-01 00:00:00

abstract：:Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

journal_title：Virology

authors： Volkman LE,Zaal KJ

更新日期：1990-03-01 00:00:00

abstract：:During the productive phase of chronic hepadnaviral infections, virion DNA synthesis occurs in the cytoplasm of the infected hepatocyte, but viral RNA is synthesized in the nucleus, apparently from a covalently closed, circular (CCC) viral DNA. J. Tuttleman, C. Pourcel, and J. Summers (1986a, Cell 47, 451-460) have sh...

journal_title：Virology

authors： Wu TT,Coates L,Aldrich CE,Summers J,Mason WS

更新日期：1990-03-01 00:00:00

abstract：:The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...

journal_title：Virology

authors： Pence DF,Davis NL,Johnston RE

更新日期：1990-03-01 00:00:00

abstract：:The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...

journal_title：Virology

authors： Parrington MA,Kang CY

更新日期：1990-03-01 00:00:00

abstract：:The interaction of parvovirus minute virus of mice (prototype strain, MVMp) with simian virus 40 (SV40)-transformed human cells (NB-E) was investigated by means of transfection with MVMp molecular clones derived from the infectious recombinant plasmid (pMM984). pMM984 inhibits stable transformation of NB-E cells to ge...

journal_title：Virology

authors： Brandenburger A,Legendre D,Avalosse B,Rommelaere J

更新日期：1990-02-01 00:00:00

abstract：:We expressed the carboxy-terminal portion of the E2 open reading frame (ORF)-encoded protein of human papillomavirus type 16 (HPV-16) and purified it to near homogeneity. Using DNase I footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (BPV-1), HPV-18, HPV-11, it binds ...

journal_title：Virology

authors： Bedrosian CL,Bastia D

更新日期：1990-02-01 00:00:00

abstract：:Foot-and-mouth disease virus protease 3C is essential for the processing of the viral precursor polyprotein. It is shown here to also inhibit gene expression in baby hamster kidney cells after transient expression from transfected cDNA fragments. Protease 3C could not be detected by indirect immunofluorescence in cont...

journal_title：Virology

authors： Tesar M,Marquardt O

更新日期：1990-02-01 00:00:00

abstract：:Genetic studies have been carried out on orbiviruses in the Great Island (GI) antigenic subgroup of the Kemerovo serogroup (Orbivrus, Reoviridae) to elucidate the functions of the 10 genomic double-stranded RNA segments. Such studies have shown that segment 4 is the major genetic determinant of neurovirulence (P.A. Nu...

journal_title：Virology

authors： Nuttall PA,Moss SR,Carey D,Jones LD,Jacobs SC

更新日期：1990-02-01 00:00:00

abstract：:Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....

journal_title：Virology

authors： Pieniazek D,Pieniazek NJ,Macejak D,Coward J,Rayfield M,Luftig RB

更新日期：1990-01-01 00:00:00

abstract：:Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...

journal_title：Virology

authors： Yeo A,Kosturka LD,Feiss M

更新日期：1990-01-01 00:00:00

abstract：:Hepatitis B virus transcripts and DNA from paired samples of neoplastic and nonneoplastic liver tissue of HBsAg seropositive patients were analyzed. The data obtained support the view that transcription of integrated DNA is frequent, both in neoplastic as well as in nonneoplastic liver tissue. In the case of one patie...

journal_title：Virology

authors： Loncarević IF,Schranz P,Zentgraf H,Liang XH,Herrmann G,Tang ZY,Schröder CH

更新日期：1990-01-01 00:00:00

abstract：:Recombinant baculoviruses containing the coding sequences of the viral structural proteins, i.e., the capsid (C) protein, the precursor to premembrane (preM) protein, and the envelope (E) protein, as well as a nonstructural protein, NS1, of Japanese encephalitis virus (JEV) were constructed. Infection of Spodoptera fr...

journal_title：Virology

authors： Matsuura Y,Miyamoto M,Sato T,Morita C,Yasui K

更新日期：1989-12-01 00:00:00

abstract：:Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...

journal_title：Virology

authors： Gerspach R,Matz B

更新日期：1989-12-01 00:00:00

abstract：:We have used field inversion gel electrophoresis to survey EBV strains for the size of the major internal repeat, IR-1, and estimate the number of 3.1-kb repeat units present. The B95-8 strain of EBV was estimated to contain 8.6 repeats. The repeat number varies considerably among naturally occurring isolates around a...

journal_title：Virology

authors： Allan GJ,Rowe DT

更新日期：1989-12-01 00:00:00

abstract：:The RTVL-H family of human endogenous retrovirus-like sequences consists of approximately 1000 "full-length" elements and at least as many solitary RTVL-H related long terminal repeats (LTRs). We have characterized cDNA clones from two human cell libraries (Hep-2 and normal peripheral blood) and have found three clone...

journal_title：Virology

authors： Mager DL

更新日期：1989-12-01 00:00:00

abstract：:To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...

journal_title：Virology

authors： Shanks M,Tomenius K,Clapham D,Huskisson NS,Barker PJ,Wilson IG,Maule AJ,Lomonossoff GP

更新日期：1989-12-01 00:00:00